Exosomes shuttle functional RNA molecules in the target cell. Increasing evidence suggests a role for exosome-derived miRNAs in the development and/or progression of specific human diseases. Pathogenic miRNAs might be exploited as novel therapeutic targets or disease biomarkers in complex diseases, including cancer. In fact, miRNAs seem to play critical roles as transcriptional and post-transcriptional regulators of epigenetic mechanisms and cell processes and have been linked to the etiology, progression and prognosis of cancer. Similar miRNA expression patterns between tumor tissue samples and circulating exosomes have been observed. HBM has developed optimized solutions for the efficient extraction of high-quality total RNA (miRNA and mRNAs) from the overall exosomes and microvesicle population or from tumor-specific exosome subpopulation, which helps to facilitate the identification of tumor miRNA or mRNAs signatures from human biofluids or cell culture media. We offer three different kits for exosome capture and RNA extraction.
EXO-TotalRNA: Overall Exosome Immunocapture and RNA extraction kit: Kit allows, by immunocapture, the isolation of overall exosome population from human biofluids or cell culture media and total RNA extraction.
TumorEXO-TotalRNA: Tumor Exosome Immunocapture and RNA extraction kit: Kit allows, by immunocapture, the enrichment of tumor-specific exosome population from human biofluids and total RNA extraction.
RNA Basic Kit: Kit allows total RNA extraction from exosomes pre-isolated with different methods (ultracentrifugation, chemical precipitation, immunocapture, size-chromatography etc.).
Applications: Direct capture and exosome RNA extraction from human biofluids and cell culture media without initial exosome purification step. Simultaneous miRNA and mRNA profiling (qRT-PCR, RT-PCR, microarray).Enrichment of tumor-derived exosomes for cancer miRNA marker profiling.
Advantages: High yield of total RNA (including small RNAs).Fast and user friendly protocol. Small starting amount of sample (less than 1 ml).Better RNA yield than similar products. The only kit on the market providing Exosome Standards as control.
Zarovni, N., Corrado, A., Guazzi, P., Zocco, D., Lari, E., Radano, G., ... & Chiesi, A. (2015). Integrated isolation and quantitative analysis of exosome shuttled proteins and nucleic acids using immunocapture approaches. Methods.