Exo-FACS

Exo-FACS allows exosome isolation from biofluids or cell culture media and FACS analysis of exosome markers. The kit consists of EXO-Prep reagent, for exosome isolation, 4 µm beads used for the overall capture of pre-isolated exosomes, lyophilized exosomes from cell culture supernatants or human biological fluids, as positive control. The characterization of exosomal proteins (membrane expressed or internal) is subsequently performed using appropriate detection antibodies against exosome associated antigens. HBM offers different Exo-FACS kits for staining of exosome markers from human biofluids (plasma, urine, serum, saliva) and from cell culture supernatants. Exo-FACS contains reagents for 20 reactions (lyophilized exosomes, beads, antibodies and buffers). Primary antibody included in the kit is against a common exosome marker (CD9 or CD63) and can be used as a positive control for protein profiling via FACS analysis.

Applications: Exosome isolation and exosome marker characterization via FACS. Exosome comprehensive profiling.

Advantages: Ready to use. No initial exosome purification required. Lyophilized Exosome Standards for positive control included. User friendly and suitable for multiple marker analyses.