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RNA Kit

How do you isolate exosomal RNA?

We offer ExoRNA™ Exosome RNA extraction kits for overall exosome immunocapture and RNA extraction as well as for Tumor-derived exosome immunocapture and RNA extraction.

Can I get enough exosomal RNA from 10 ml cell culture supernatant for qPCR?

Exosome yield depends on the cell confluence, cell type, health of the cells and time of harvest, but in general yes, 10 ml are enough for getting a good yield of RNA for qPCR, by using our Exo-totalRNA isolation kit.

Regarding the lyophilized exosome standards, do you also the characterize their DNA/RNA contents too in addition to the particle number and the protein content?

Lyophilized standards are currently used as a positive control in RNA and DNA kit, so it is possible to use them for isolation of nucleic acids, although each lot has characterized for protein content and particle number (NTA). In addition, many customers are using as mutation control in spike-in experiments.

Can you recommend a miRNA isolation kit for qPCR analysis?

We offer Exo-totalRNA extraction kit. The kit contains immunobeads for overall exosomes capture, all the solutions, columns and elution tubes for RNA extraction. This kit allows high yield of total RNA extraction, including small RNAs, miRNAs.

Why is the exosomal miRNA levels higher in plasma as compared to the serum exosomal miRNA?

Plasma contains a higher exosome amount than serum, since during the serum preparation exosomes can remain entrapped into the clot, thus even exosomal miRNAs are higher in plasma than in serum.

Can you recommend a miRNA isolation kit for qPCR analysis?

We offer Exo-totalRNA extraction kit. The kit contains immunobeads for overall exosomes capture, all the solutions, columns and elution tubes for RNA extraction. This kit allows high yield of total RNA extraction, including small RNAs, miRNAs.

Why is the exosomal miRNA levels higher in plasma as compared to the serum exosomal miRNA?

Plasma contains a higher exosome amount than serum, since during the serum preparation exosomes can remain entrapped into the clot, thus even exosomal miRNAs are higher in plasma than in serum.

How can I normalize mRNA expression in exosomes?

You can use miRNA genes such as miRNA16, miRNA26a, miRNA221, miRNA22, miRNA181a, miRNA181c, miRNA103, miRNA191, let7d and small RNAs (5SrRNA and U6snRNA) that is normally expressed in exosomes.