Lyophilized Exosomes and Microvesicles are obtained from a variety of biological sources: cell culture supernatant, human plasma, serum, urine and saliva of pool of healthy donors. Exosomes (40-120 nm) are purified following a combination of tangential flow filtration (TFF) and size exclusion chromatography (SEC). Microvesicles (150- 1000 nm) are separated by tangential flow filtration using the TFF-MV filter, then purified by SEC. Isolated vesicles are quantified and validated for overall protein content, size distribution and particle number by NTA (Nanoparticles Tracking Analysis) with Zetaview analyzer (Particle Metrix). Effects of lyophilization on stability of EV proteins are comparable to other storage methods such as storing fresh exosomes at -20C. Lyophilized exosomes are easy to store and ship and are stable for long term (up to 36 months).
Fluorescent labeled Exosome and Microvesicles: Upon request, HBM can also provide fluorescent labeled Exosome Standards from human biofluids or cell media for EV tracking in fluorescence microscopy experiments.
Applications: Assay calibration, Control (spike-in) for EV quantification, protein marker analysis using different techniques. Extraction and analysis of EV associated nucleic acids. Flow cytometry. Electron microscopy.
Advantages: Highly pure, easy to reconstitute, easy to ship and store (+4°C), Long term storage stability (36 months). EVs are available from a large cell line bank (over 100 cell lines) on request.