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Exosome Standards

Exosome Standards

HansaBioMed Life Sciences lyophilized exosomes were obtained from different biological sources that includes exosomes from human biofluids of certified healthy donors (plasma, serum, urine, saliva) and from different cell culture media. Exosome isolation is obtained through a combination of ultracentrifugation and microfiltration procedures. Exosomes are subsequently quantified and validated for overall protein content and particle number by NTA with Nanosight. Effects of lyophilization on stability of exosome proteins are comparable to other storage methods such as storing fresh exosomes at -20C. Lyophilized exosomes are easy to store and ship and are stable for long term (up to 36 months).

Fluorescent labeled Exosome Standards: Upon request, HBM can also provide fluorescent labeled Exosome Standards from human biofluids or cell media for EV tracking in fluorescence microscopy experiments.

Applications: Assay calibration, Control (spike-in) for exosome quantification, protein marker analysis using different techniques. Extraction and analysis of exosome nucleic acid. Standardized positive controls for immunocapture performance evaluation. Flow cytometry. Electron microscopy.

Advantages: Highly pure, easy to reconstitute, easy to ship and store (+4°C), Long term storage stability (36 months). Exosomes are available from a large cell line bank (over 100 cell lines) on request.

References:

Valkonen, S., van der Pol, E., Böing, A., Yuana, Y., Yliperttula, M., Nieuwland, R., ... & Siljander, P. R. M. (2016). Biological reference materials for extracellular vesicle studies. European Journal of Pharmaceutical Sciences.

Oliveira-Rodríguez, M., López-Cobo, S., Reyburn, H. T., Costa-García, A., López-Martín, S., Mo, M. Y., ... & Blanco-López, M. C. (2016). Development of a rapid lateral flow immunoassay test for detection of exosomes previously enriched from cell culture medium and body fluids. Journal of Extracellular Vesicles, 5.

Arcangeletti, M. C., Simone, R. V., Rodighiero, I., De Conto, F., Medici, M. C., Maccari, C., ... & Calderaro, A. (2016). Human cytomegalovirus reactivation from latency: validation of a “switch” model in vitro. Virology Journal, 13(1), 179.

Zhang, Peng, Mei He, and Yong Zeng. "Ultrasensitive Microfluidic Analysis of Circulating Exosomes Using Nanostructured Graphene Oxide/Polydopamine Coating." Lab on a Chip (2016).

Sitar, S., Kejžar, A., Pahovnik, D., Kogej, K., Tušek-Žnidarič, M., Lenassi, M., & Žagar, E. (2015). Size characterization and quantification of exosomes by asymmetrical-flow field-flow fractionation. Analytical chemistry, 87(18), 9225-9233.

Gardiner, C., Shaw, M., Hole, P., Smith, J., Tannetta, D., Redman, C. W., & Sargent, I. L. (2014). Measurement of refractive index by nanoparticle tracking analysis reveals heterogeneity in extracellular vesicles. Journal of extracellular vesicles, 3.

Ferrante, S. C., Nadler, E. P., Pillai, D. K., Hubal, M. J., Wang, Z., Wang, J. M., ... & Freishtat, R. J. (2014). Adipocyte-derived exosomal miRNAs: a novel mechanism for obesity-related disease. Pediatric research, 77(3), 447-454.

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