HBM Immunoplates are 96 multiwell plates covalently pre-coated with specific exosome-binding antibodies allowing exosome capture and isolation from different sources (cell supernatant, human plasma, serum, urine and saliva). Covalent coating improves the correct orientation of antibodies maximizing the quantity of immunocaptured exosomes and increasing the binding efficiency of the plate. In addition the coating chemistry reduces the aspecific binding of circulating protein complexes and cell debries, and it helps the enrichment of exosome subpopulations. HBM has developed different types of plates for capturing the overall or enriching specific exosome subpopulations (tumor, neural, glial, monocytes and platelets). Plates are blocked and stabilized for long term storage.
Applications: ELISA immunoplates allow multiple profiling of exosome markers from a single sample or screening of a large number of samples. Exosome capture and quantification from human biofluids (plasma, serum, urine, saliva) and from human or mouse cell media. Enrichment in specific exosome subpopulations (neural, glial, tumor derived). Titration of purified exosomes. Immunoplates are also suitable for RNA extraction from immunocaptured exosomes.
Advantages: Immunoplates are ready to use and stable for long term (up to 2 years). No exosome pre-purification is required. Small amount of sample required (100 μl per well). Open platform for custom coating solutions.