HBM provides several types of Immunobeads for capturing and isolating overall or specific exosome sub-populations. Latex immunobeads are covalently coupled with antibodies against exosome surface antigens, allowing exosome capture from human biofluids (tested for plasma, serum and urine) and cell culture media without pre-purification steps (ultracentrifuge or other method for exosome purification). HBM immunobeads are able to capture the overall exosome population (Immunobeads for Overall Exosome capture) or to enrich exosome subpopulation derived from tumor source (Tumor-derived exosome capture and enrichment). Immunobeads are supplied with an Exosome Elution Buffer, that allows detachment and elution of captured exosomes for downstream analyses, and with a Beads Regeneration Buffer to regenerate immunobeads for further usage. All latex Immunobeads are available in two sizes (0.4 and 1 micron of diameter) and are sold in packages of 10 and 20 reactions, or as TEST format including material for 3 and 5 reactions.
Applications: Overall exosome isolation from cell culture media and human biofluids (tested for plasma, serum, urine). Overall exosome isolation from mouse biofluids (tested for plasma and serum). Capture and enrichment of human exosome subpopulation (tumor-derived). Immunobeads are suitable for nucleic acid extraction, protein profiling of exosome markers, exosome elution from immunobeads.
Ready to use.
Easy, fast and efficient protocol.
Small sample volume of biofluid or cell culture medium. No ultracentrifugation or other methods for exosome purification required. Supplied with buffer for exosome elution from beads. Immunobeads can be regenerated with Beads Regeneration Buffer and reused.