EXO-Prep is a fast and convenient method of EV isolation from biofluids and cell culture supernatants. Isolation with EXO-Prep is based on chemical precipitation. Samples are incubated with EXO-Prep solution on ice, so that vesicles will precipitate following centrifugation. Isolated EVs are suitable for a wide range of analyses, such as NTA, protein profiling by using different techniques (western blotting, ELISA, FACS), nucleic acid extraction and profiling of mRNA or small RNA markers.
Applications: Single step isolation of EVs from biofluids and cell supernatants. Isolated EVs can be used for NTA analysis, protein profiling (WB, FACS, ELISA) and nucleic acid (RNA and DNA) extraction and profiling.
Advantages: Easy and fast protocol. Pellet can be easily resuspended in PBS. EXO-Prep is able to isolate exosome from small volumes of sample (as low as 100 μl of plasma).