Size Exclusion Chromatography (SEC) is considered one of the best methods for isolating and purifying exosomes and extracellular vesicles (EVs) from different matrices. In particular, this technique is very efficient for separating exosomes from the circulating proteins and does not affect the original shape and functionality of the vesicles. HBM has developed different classes of SEC columns for EV purification: PURE-EVs and miniPURE-EVs are two different size of columns for EV purification from small volume (2 ml up to 100 μl) of different matrices. In addition, HBM provides PURE-EVs PLUS, which, together with the SEC column, includes MWCO (molecular weight cut-off) concentrators especially designed for concentrating the EV preparation. Using PURE-EVs PLUS you can get in approximately 15 minutes a concentrate EV preparation.
Applications: Exosome isolation from biofluids and cell media. Purification of pre-isolated EVs from contaminants Multiple analysis of isolated exosomes (NTA, ELISA, FACS, WB, EM, MS, nucleic acid extraction, etc).
Advantages: Easy and fast protocol (turnaround time approximately 15 minutes). Isolate exosome from small volumes of sample. PURE-EVs PLUS includes MWCO concentrators especially designed for EV concentration. Reusable up to 5 times. Easy to store and ship (4°C).